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The intracellular effects of manuka honey on Staphylococcus aureus.

The intracellular effects of manuka honey on Staphylococcus aureus.









No. Divisions/Titles for Abstract Details

1 Abstract Title The intracellular effects of manuka honey on Staphylococcus aureus.


2 Abstract Source European Journal of Clinical Microbiology & Infectious Diseases, 29, 45.


3 Abstract Author(s) Henriques, A. F., Jenkins, R. E., Burton, N. F. & Cooper, R. A.


4 Article Affiliation Instituto de Biologia Molecular e Celular, Group of Molecular BiologyUniversidade do PortoPortoPortugal.


5 Abstract The purpose of this study was to investigate the effect of manuka honey on Staphylococcus aureus (a gram-positive, round-shaped bacterium that is a member of the Firmicutes, and is frequently found in the nose, respiratory tract, and on the skin.)in order to identify the intracellular (located or occurring within a cell or cells.) target site. The mode of inhibition of manuka honey against S. aureus NCTC 10017 was investigated by determining the minimum inhibitory concentration (MIC) (the lowest concentration of a chemical which prevents visible growth of a bacterium), minimum bactericidal concentration (MBC) (the lowest concentration of an antibacterial agent required to kill a particular bacterium) and the effect of time on viability (ability to survive or live successfully). Structural changes were observed by scanning (SEM) (a type of electron microscope that produces images of a sample by scanning the surface with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition)and transmission electron microscopy (TEM) ((TEM, also sometimes conventional transmission electron microscopy or CTEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid)of cells suspended for 4 h at 37°C in 0.05 mM Tris buffer (an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa) containing 10% (w/v) manuka honey and were compared to cells in buffer alone or buffer containing 10% (w/v) artificial honey (to assess osmotic damage). A bactericidal(to kill bacteria) mode of inhibition for manuka honey on S. aureus was established. Marked structural changes in honey-treated cells were seen only with TEM, where a statistically significant increase in the number of whole cells with completed septa compared to untreated cells were observed (P < 0.05). Structural changes found with TEM suggest that honey-treated cells had failed to progress normally through the cell cycle and accumulated with fully formed septa at the point of cell division without separating. Sugars were not implicated in this effect. The staphylococcal target site of manuka honey involves the cell division machinery.


6 Summary Structural changes found with TEM suggest that honey-treated cells had failed to progress normally through the cell cycle and accumulated with fully formed septa at the point of cell division without separating. Sugars were not implicated in this effect. The staphylococcal target site of manuka honey involves the cell division machinery.


7 Article Published Date January 2010.


8 Study Type Invitro.


9 Substances manuka honey.


10 Diseases bacterial infection.


11 Pharmacological Actions anti-bacterial.


12 Link https://goo.gl/HgdKrX








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