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Olea europaea leaf extract alters microrna expression in human glioblastoma cells.

Olea europaea leaf extract alters microrna expression in human glioblastoma cells.








No. Divisions/Titles for Abstract Details

1 Abstract Title Olea europaea leaf extract alters microrna expression in human glioblastoma cells.


2 Abstract Source Journal of cancer research and clinical oncology, 138, 1831-1844.


3 Abstract Author(s) Tunca, B., Tezcan, G., Cecener, G., Egeli, U., Ak, S., Malyer, H., Tumen, G. & Bilir, A.


4 Article Affiliation Department of Medical Biology, Medical Faculty, Uludag University, Bursa, Turkey.


5 Abstract Purpose
Glioblastoma multiforme (GBM) is the most common and the most lethal form of primary malignant tumors in the central nervous system. There is an increasing need for the development of more efficient therapeutic approaches for the treatment of these patients. One of the most attractive cancer therapy methods to date is the induction of tumor cell death by certain phytochemicals. Interestingly, bioactive compounds have been shown to alter micro RNA (miRNA) expression involved in several biological processes at the posttranscriptional level. The present study aimed to evaluate whether Olea europaea leaf extract (OLE) has an anticancer effect and modulates miRNA expression in GBM.

Materials and methods
Firstly, the anti-proliferative activity of OLE and the nature of the interaction with temozolomide (TMZ) of OLE were tested in human glioblastoma cell line T98G cells by trypan blue and WST-1 assays and than realized miRNA PCR array analysis. Potential mRNA targets were analyzed bioinformatically.

Results
OLE exhibited anti-proliferative effects on T98G cell lines. Cells were treated with temozolomide (TMZ) in the presence OLE, and changes to miRNA expression levels were identified by PCR array analysis. miRNA target genes are involved in cell cycle and apoptotic pathways. Specifically, miR-181b, miR-153, miR-145, miR-137, and let-7d were significantly upregulated after treatment with both TMZ and OLE.

Conclusion
Our results suggest that OLE modulates the expression of some miRNAs related to anticancer activity in GBM and the response to TMZ. Further studies and validations are needed, but we suggest that OLE might be used for in vivo studies and future medical drug studies.


6 Summary -


7 Article Published Date
November 2012.


8 Study Type Invitro.


9 Substances Olive leaf.


10 Diseases Cancer.


11 Pharmacological Actions Anticancer.


12 Link https://goo.gl/1gfkat








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