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In vitro antioxidant and free radical scavenging studies of alcoholic extract of Medicago sativa L.

In vitro antioxidant and free radical scavenging studies of alcoholic extract of Medicago sativa L.









No. Divisions/Titles for Abstract Details

1 Abstract Title In vitro antioxidant and free radical scavenging studies of alcoholic extract of Medicago sativa L.


2 Abstract Source Romanian Journal of Biology-Plant Biology, 55, 15-22.


3 Abstract Author(s) Rana, M., Katbamna, R., Padhya, A., Dudhrejiya, A., Jivani, N. & Sheth, N.


4 Article Affiliation Department of Pharmacognosy, S. J. Thakkar pharmacy College, Rajkot.


5 Abstract Free radicals (Free radicals are atoms or groups of atoms with an odd (unpaired) number of electrons and can be formed when oxygen interacts with certain molecules.)are implicated for many diseases including diabetes mellitus, ( commonly referred to as diabetes, is a group of metabolic disorders in which there are high blood sugar levels over a prolonged period)arthritis,(is inflammation of one or more of your joints. The main symptoms of arthritis are joint pain and stiffness, which typically worsen with age) cancer,(is a group of diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. These contrast with benign tumors, which do not spread to other parts of the body) ageing. (is the process of becoming older. The term refers especially to human beings, many animals, and fungi, whereas for example bacteria, perennial plants and some simple animals are potentially immortal)etc. In the treatment of these diseases, antioxidant (a substance that inhibits oxidation, especially one used to counteract the deterioration of stored food products)therapy has gained utmost importance. Alcoholic extract of Medicago sativa was studied for its in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, (a chemical substance added to a mixture in order to remove or de-activate impurities and unwanted reaction products, for example oxygen, to make sure that they will not cause any unfavorable reactions)iron chelating activity, lipid peroxidation (the oxidative degradation of lipids. It is the process in which free radicals "steal" electrons from the lipids in cell membranes, resulting in cell damage. This process proceeds by a free radical chain reaction mechanism.)assay, nitric oxide scavenging assay, and alkaline DMSO assay. The results were analyzed statistically by the regression method. Its antioxidant activity was estimated by IC50 value and the values are 100.38 μg/ml (DPPH radical scavenging), 12.33 μg/ml (ABTS radical scavenging), 115.79 μg/ml (Iron chelating activity) and 49.06 μg/ml (lipid peroxidation), 21.77 μg/ml (nitric oxide scavenging) and 15.91 μg/ml (alkaline DMSO). In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Medicago sativa is effective against free radical mediated disease.


6 Summary In all the testing, a significant correlation existed between concentrations of the extract and
percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation.
The antioxidant property may be related to the polyphenols and flavonoids present in
the extract. These results clearly indicate that Medicago sativa is effective against free
radical mediated disease.


7 Article Published Date 2010.


8 Study Type invitro.


9 Substances alfalfa.


10 Diseases oxidation.


11 Pharmacological Actions antioxidant.


12 Link https://goo.gl/yHyDgJ








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