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In vitro Anti- Helicobacter pylori Effects of Sweet Basil ( Ocimum basilicum L.) and Purple Basil ( Ocimum basilicum var. purpurascens ).

In vitro Anti- Helicobacter pylori Effects of Sweet Basil ( Ocimum basilicum L.) and Purple Basil ( Ocimum basilicum var. purpurascens ).










No.Divisions/Titles for AbstractDetails

1Abstract TitleIn vitro Anti- Helicobacter pylori Effects of Sweet Basil ( Ocimum basilicum L.) and Purple Basil ( Ocimum basilicum var. purpurascens ).


2Abstract SourcePakistan Journal of Biological Sciences.


3Abstract Author(s)Nakhaei, M. M., Malekzadeh, F., Khaje-Karamoddin, M. & Ramezani, M. 


4Article AffiliationDepartment of Medical Microbiology, Ghaem Hospital, School of Medicine.


5AbstractAnti- Helicobacter pylori (Helicobacter pylori, previously Campylobacter pylori, is a gram-negative, microaerophilic bacterium found usually in the stomach)effects of leaves of Ocimum basilicum L. (sweet basil, Lamiaceae) and Ocimum basilicum var. purpurascens (purple basil, Lamiaceae) were evaluated. Macerated aqueous and methanol extracts were tested against 45 clinical isolates of Helicobacter pylori using paper Disc Diffusion Method (DDM) (disc diffusion antibiotic sensitivity testing) is a test of the antibiotic sensitivity of bacteria)on modified egg yolk emulsion agar (EYE agar). Although there were small differences in sensitivity among the isolates tested, but all isolates were susceptible to methanol and aqueous extracts. The Minimum Inhibitory Concentrations (MIC) (the lowest concentration of an antimicrobial (like an antifungal, antibiotic or bacteriostatic) drug that will inhibit the visible growth of a microorganism after overnight incubation.)of the extracts from leaves of sweet basil and purple basil were 677 and 729 μg mL<SUP>-1</SUP>, respectively using agar dilution method.(The agar diffusion test (Kirby–Bauer antibiotic testing, KB testing, or disc diffusion antibiotic sensitivity testing) is a test of the antibiotic sensitivity of bacteria. It uses antibiotic discs to test the extent to which bacteria are affected by those antibiotics.) Antibacterial constituents of sweet basil extract were stable after one month of storage at 4°C but lost 31% its activity after 4 weeks at room temperature. The extract preserved its activity when heated to 80°C for 30 min and autoclaved at 121°C for 20 min. It was stable at pH 5-8.


6SummaryAnti- Helicobacter pylori effects of leaves of Ocimum basilicum L. (sweet basil, Lamiaceae) and Ocimum basilicum var. purpurascens (purple basil, Lamiaceae) were evaluated. Macerated aqueous and methanol extracts were tested against 45 clinical isolates of Helicobacter pylori using paper Disc Diffusion Method (DDM) on modified egg yolk emulsion agar (EYE agar). Although there were small differences in sensitivity among the isolates tested, but all isolates were susceptible to methanol and aqueous extracts. The Minimum Inhibitory Concentrations (MIC) of the extracts from leaves of sweet basil and purple basil were 677 and 729 μg mL<SUP>-1</SUP>, respectively using agar dilution method. Antibacterial constituents of sweet basil extract were stable after one month of storage at 4°C but lost 31% its activity after 4 weeks at room temperature. The extract preserved its activity when heated to 80°C for 30 min and autoclaved at 121°C for 20 min. It was stable at pH 5-8. 


7Article Published Date2007.

8Study Typeinvitro.


9SubstancesBasil.


10DiseasesIBS.

11Pharmacological ActionsTreatment of IBS.


12Linkhttps://goo.gl/dedTC2








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