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Identification of phenolic compound in manuka honey as specific superoxide anion radical scavenger using electron spin resonance (ESR) and liquid chromatography.

Identification of phenolic compound in manuka honey as specific superoxide anion radical scavenger using electron spin resonance (ESR) and liquid chromatography.









No. Divisions/Titles for Abstract Details

1 Abstract Title Identification of phenolic compound in manuka honey as specific superoxide anion radical scavenger using electron spin resonance (ESR) and liquid chromatography with coulometric array detection.


2 Abstract Source Journal of the Science of Food and Agriculture, 85, 872-878.


3 Abstract Author(s) Inoue, K., Murayama, S., Seshimo, F., Takeba, K., Yoshimura, Y. & Nakazawa, H.


4 Article Affiliation Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.


5 Abstract Apitherapy (is a branch of alternative medicine that uses honey bee products including honey, pollen, propolis, royal jelly and bee venom)has become the focus of attention as a form of folk and preventive medicine for treating certain conditions and diseases as well as promoting overall health and well-being. In apitherapy, honey is the therapeutic (relating to the healing of disease)agent used for dressing surgical wounds, burns or skin ulcers(are open sores that develop on the inner  lining of the organ ), as well as for dyspepsia (common condition and usually describes a group of symptoms rather than one predominant symptom), peptic ulcer ( painful sores in the lining of the stomach, esophagus, or small intestine), etc., because of its antioxidant (a substance that inhibits oxidation, especially one used to counteract the deterioration of stored food products)activity. Therefore, it is important to determine the antioxidants in honey by analytical techniques. In the present study, the antioxidant activities of honeys from different floral sources were investigated by electron spin resonance (method for studying materials with unpaired electrons)(1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2/NaOH/DMSO scavenging systems), liquid chromatography with coulometric array detection (LC-ED), and liquid chromatography ( technique in analytical chemistry used to separate, identify, and quantify each component in a mixture)with electrospray mass spectrometry (LC-MS)(a desorption ionization method. ). The antioxidant activities of some unifloral honeys (acacia, Chinese milk vetch, buckwheat and manuka) were evaluated using the radical scavenging ( is a chemical substance added to a mixture in order to remove or de-activate impurities and unwanted reaction products, for example oxygen, to make sure that they will not cause any unfavorable reactions)systems. It was shown that DPPH radical scavenging activity was significantly different among the honeys, with buckwheat and manuka honeys having significantly higher scavenging activity than acacia honey. In addition, only manuka honey had specific scavenging activity for superoxide anion radicals. The compound responsible for this activity in manuka honey was identified by LC-ED and LC-MS. Careful examination of the LC-ED chromatographic patterns of manuka and other honey samples revealed a distinct peak in the chromatogram of manuka honey to be methyl syringate (A benzoate ester obtained by the formal condensation of carboxy group of syringic acid with methanol.)(MSYR). The radical scavenging activity of MSYR was specific for superoxide anion radicals, similar to the case of manuka honey. Copyright © 2005 Society of Chemical Industry.


6 Summary The antioxidant activities of some unifloral honeys (acacia, Chinese milk vetch, buckwheat and manuka) were evaluated using the radical scavenging systems. It was shown that DPPH radical scavenging activity was significantly different among the honeys, with buckwheat and manuka honeys having significantly higher scavenging activity than acacia honey. In addition, only manuka honey had specific scavenging activity for superoxide anion radicals. The compound responsible for this activity in manuka honey was identified by LC-ED and LC-MS. Careful examination of the LC-ED chromatographic patterns of manuka and other honey samples revealed a distinct peak in the chromatogram of manuka honey to be methyl syringate (MSYR). The radical scavenging activity of MSYR was specific for superoxide anion radicals, similar to the case of manuka honey.


7 Article Published Date
26 January 2005.


8 Study Type Invitro.


9 Substances Manuka honey.


10 Diseases Oxidative demage.


11 Pharmacological Actions Antioxidant.


12 Link https://goo.gl/XCT1F1








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