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Evaluation of Anticancer Activity of Water and Juice Extracts of Young Hordeum Vulgare in Human Cancer Cell Lines HT-29 and A549.

Evaluation of Anticancer Activity of Water and Juice Extracts of Young Hordeum Vulgare in Human Cancer Cell Lines HT-29 and A549.

No. Divisions/Titles for Abstract Details

1 Abstract Title Evaluation of Anticancer Activity of Water and Juice Extracts of Young Hordeum Vulgare in Human Cancer Cell Lines HT-29 and A549.

2 Abstract Source Ann Agric Environ Med, 24, 345-349.

3 Abstract Author(s) Czerwonka, A., Kawka, K., Cykier, K., Lemieszek, M. K. & Rzeski, W.

4 Article Affiliation Departament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University.

5 Abstract Introduction and objective. Barley (Hordeum vulgare L.) is known as a rich source of different bioactive(Having an effect upon a living organism, tissue, or cell) compounds. At present, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals, vitamins, carbohydrates, amino acids, phenolic compounds and proteins. It is possible that the composition of chemical ingredients beneficial for health may induce an anticancer potential of young barley in human colon adenocarcinoma(A cancer that develops in the lining or inner surface of an organ and usually has glandular (secretory) properties) (HT-29) and human lung adenocarcinoma (A549) cell lines. Materials and method. Hordeum vulgare water extract (HWE) and Hordeum vulgare juice extract (HJE) were prepared. Cell proliferation and viability were examined with the use of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and NR (neutral red) methods. Induction of necrosis(The death of living cells or tissues) was assessed by propidium iodide/Hoechst staining. Progress of the cell cycle(The sequence of events within the cell between mitotic (cell) divisions) involved in cell proliferation, apoptosis(A form of cell death in which a programmed sequence of events leads to the elimination of cells without releasing harmful substances into the surrounding area), and regulation of transcription(Making an RNA copy from a sequence of DNA (a gene)) was estimated using flow cytometry analysis. Additionally, the capability of free radical(an especially reactive atom or group of atoms that has one or more unpaired electrons) scavenging was evaluated with the DPPH assay. Results. The study revealed that extracts inhibited the proliferation of cancer cells. The NR study confirmed the low cytotoxic activity(Any agent or process that kills cells) of the tested extracts to normal human colon epithelial(a membranous cellular tissue that covers a free surface or lines a tube or cavity of an animal body and serves especially to enclose and protect the other parts of the body, to produce secretions and excretions, and to function in assimilation) cells (CCD 841 CoTr) and human skin fibroblasts(A type of cell found in connective tissue throughout the body that produces collagen and other proteins found in the extracellular (between cells) spaces) (HSF). Furthermore, a dose-dependent cytotoxicity against HT-29 cells, but not A549 cells, has been reported. The free radical scavenging activity was observed in the case of the HWE but not the HJE. Conclusions. The obtained results indicate a cancer chemopreventive potential of young barley as a safe dietary agent in colon carcinoma.

6 Summary Barley grass induces cancer cell proliferation inhibition, necrosis induction, and noticeable antioxidant activity.

7 Article Published Date June 2017.

8 Study Type Invitro.

9 Substances Barley grass.

10 Diseases Cancer.

11 Pharmacological Actions Anticancer.

12 Link

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