• الأردن: 0096278140993 - 00962790633919, الإمارات: 00971564999475 - 00971501299947 - 00971564880709
  • الجزائر: 0549996833, تونس: 0021671805607, بريطانيا: 00447517362128 - 00447519398225 - 00447519398239
  • تركيا: 0095539440472, أمريكا: 0017736162627 - 0017735728151 - 0018472850055

The world's biggest scientific documented health website for the happiness and health of your family, with science and faith we build the balance

93979218

Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells.

Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells.









No. Divisions/Titles for Abstract Details

1 Abstract Title Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells.


2 Abstract Source Nutrition Research, 29, 275-280.


3 Abstract Author(s) An, M. J., Cheon, J. H., Kim, S. W., Park, J. J., Moon, C. M., Han, S. Y., Kim, E. S., Kim, T. I. & Kim, W. H.


4 Article Affiliation Department of Internal Medicine and Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.


5 Abstract Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)–1β with or without bovine colostrum. The effects of colostrum on nuclear factor κB (NF-κB) signaling in HT-29 cells were examined using real-time reverse transcriptase–polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-κB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-κB–α, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1β-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1β-induced NF-κB activation, including NF-κB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-κB–α degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-κB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation.


6 Summary Moreover, it suppressed IL-1β-induced NF-κB activation, including NF-κB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-κB–α degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-κB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation.


7 Article Published Date 01/05/2009.


8 Study Type Invitro.


9 Substances Colostrum.


10 Diseases Inflammation.


11 Pharmacological Actions Antiinflammatory.


12 Link https://goo.gl/N8A1qh








videos balancecure


Share it



Subscribe with our newsletter


Comments

No Comments

Add comment

Made with by Tashfier

loading gif
feedback